This page displays all the Research Images from the 2013 GSLS Poster & Image Competition.
Follow the links below for the other part of the competition:
1st Prize - Mubeen Goolam.
The first four days of mouse embryonic development are dedicated to segregating and specifying the cells of the embryo into three distinct lineages. This microphotograph, taken with a Leica SP5 confocal microscope, shows cultured mouse E4.5 embryos that were stained with fluorescent antibodies to identify the different cell lineages. The light blue cells are the trophectoderm which will form part of the placenta, The navy cells are the pluripotent epiblast, which will go on to form all the cells of the embryo proper, and the pink cells are the primitive endoderm which will differentiate into the yolk sac.
2nd Prize - Xana Almeida.
Polychrome zebrafish. To investigate the mechanisms of retinal development in vivo, we generated this polychrome transgenic fish. All major retinal neurons are marked with fluorescent proteins, which are driven by three different promoters. Crx promoter drives the expression of CFP in photoreceptor and bipolar cells. Ath5 drives the expression of RFP in retinal ganglion cells, which project their axons into the brain. Ptf1a drives the expression of GFP in horizontal and amacrine cells, as well as in spinal chord neurons. The image was produced by stitching 15 individual panels captured using a laser scanning confocal microscope.
3rd Prize - Nuri Purswani.
This image is representing an air pore structure. Air pores are repeating multicellular complexes found on the epidermis of liverworts. Their main biological functions are gas exchange, photosynthesis and water uptake. Air pores have a similar appearance to the stomatal pores found in leaves although their evolutionary origin is different. Liverworts are haploid plants and have very simple morphologies. It is thought that the early terrestrial plants displayed a similar appearance to liverworts. Liverworts are ideal models for answering developmental biology questions due to the high speed of genetic manipulation compared to other plants and their easy accessibility for live imaging from the early embryonic stages. In this image, the cell membranes and nuclei have been marked with green and red fluorescent proteins respectively (green-GFP, red-RFP). Imaging conditions: This image is a two-channel average intensity projection of a three-dimensional cellular structure. It was taken with a Leica SP5 confocal microscope using a 40X oil immersion objective and sequential excitation parameters of 488 nm (GFP) and 564 nm (RFP).
The image, taken using a confocal laser scanning microscope, illustrates clustering of the immune cells around an alveolus in a mouse lung during allergic inflammation. Cytokine interleukin-13 secreting cells are shown in green and are now known to be a novel cell type called innate lymphoid cells 2. They are depicted to be the major producers of interleukin-13 at this time point of the immune response in contrast to the previous belief that T cells, stained with a red fluorophore-conjugated antibody directed against CD3, secreted the bulk of the cytokine. Blue nuclear stain was used to portray general tissue architecture.
To cope with contamination many insects have developed cleaning devices on their forelegs which are used for grooming the antennae. In Camponotus rufifemur ants, the antenna cleaner consists of a notch on the tarsus facing a spur at the end of the tibia. Both notch and spur feature comb and brush-like structures. During a natural cleaning movement an antenna is pulled through a clamp formed by the tibial spur and the tarsal notch. For this SEM image I arranged the antenna and the cleaning device in the position observed under natural conditions. I am a 2nd year PhD student in the "Workgroup of Insect Biomechanics" (Department of Zoology). The picture was taken with a field emission gun scanning electron microscope (FEG-SEM) and the scale bar length is 100 µm.
"Colour vision". During development, multipotent progenitor cells proliferate and differentiate to form all neuronal subtypes in the retina. By combining in vivo imaging with genetic labelling we are able to visualize the process of retina development at the cellular level in real time. The image, taken by a confocal microscope, depicts a developed eye of a quadruple transgenic zebrafish. The combinatorial expression of GFP, RFP, membrane GFP and CFP, under the promoters of Lhx1, Ptf1a, Ath5 and Crx respectively, is unique for each cell type allowing us to determine the fate of cells as they are being born.
The image shows a confocal immunofluorescence staining of a late Drosophila melanogaster embryo. The fly embryo outline is depicted in blue decorating actin, a protein present in all cells. In red is shown collagen, an extracellular matrix component. This protein is produced and secreted by highly motile cells called hemocytes (red positive cells). During development hemocytes migrate all over the Drosophila embryo to deposit collagen over different organs such as the digestive tube, muscles and the central nervous system, enabling the embryo to properly develop.
The image shows a confocal image of a very early fly Drosophila melanogaster embryo stained with a fluorescent antibody. Like the eggs of other insects, but unlike vertebrates, it begins its development in an unusual way: a series of nuclear divisions without cell division creates a syncytium. About 15 of the most posterior nuclei segregate into the germ-line precursors cells (the so called pole cells, green) that will give rise to eggs or sperm. The image shows how plasma membranes (pink) grow inward from the egg surface to enclose each nucleus, thereby converting the syncytial blastoderm into a cellular blastoderm.
This image is an optimising step in my work using Saccharomyces cerevisiae looking at the basics; to see the difference between cells which were grown normally (far left), with severe heat shock (second to far left) and those which had pre-treatment of sub-lethal heat shock. The three far right were technical replicates. We wanted to see that the cells with pre-treatment had higher chance of surviving the later heat shock than those without. By accident I also scanned my hand as I was scanning the plate. But I think it, although done by accident, symbolises how man can study complex questions, like the eukaryotic stress response, in simple systems such as yeast. Translating into a better understanding of human diseases related to stress, working towards a future where more refined therapeutic treatments can be designed based on what we know about the fundamental elements which cause disease in the first place.
Differences in the cytoskeleton of cultured endothelial progenitor cells (EPCs) extracted from the blood of volunteers diagnosed with pulmonary arterial hypertension with a known mutation in the bone morphogenic protein receptor type II. EPCs were stained for α-tubulin (green), actin (red) and DNA (blue) using the indirect immunofluorescence technique and pictures were acquired using a confocal microscope with a 40X objective. EPCs from healthy volunteers showed organised microtubules, whilst a significant proportion of EPCs from patients with pulmonary arterial hypertension have disorganised and disrupted microtubules, as it can be seen in the picture.
The image above shows one of my adherent growing, self-renewing cell lines, freshly derived from a patient brain tumour sample (Glioblastoma multiforme (GBM)). The cells above were cultivated under serum free conditions & under addition of growth factors. Cells of the line in question (A13) were stained for the expression of different surface and membrane molecules relevant for invasive capabilities of the cells. The image above shows a co-staining for two of those molecules, differently modified, after acquisition with a fluorescent microscope, to bring out different intensities of the staining or to enhance the visibility of the processes.
This image depicts a neutrophil that has extruded a web of chromatin loaded with antimicrobial proteins, a so-called neutrophil extracellular trap (NET) in response to culture with eosinophil supernatants. These have been stained with the nuclear dye sytox green and this process is believed to be a key mechanism of how neutrophils trap and kill bacterial, fungal and protozoan pathogens. Neutrophils are only 10 µM in diameter but this vast meshwork extends over a vast area, ultimately helping to prevent dissemination of pathogens throughout the body. However, this strategy has consequences since the antimicrobial proteins are highly cytotoxic and can result in significant collateral damage to neighbouring tissues.
Visualization of the Drosophila blood brain barrier. The image shows a membrane reporter that is expressed by a gene required for the Drosophila blood brain barrier. The visualisation highlights areas with strong expression in yellow, while weaker expression is shown in red and blue. Strong expression can be found in central areas, where the airways enter the central nervous system. The images was stained with Immunohistochemistry and acquired with a Leica SP5 confocal microscope.
Confocal micrograph depicting the 3-D culture of human hepatocytes derived from induced pluripotent stem cells (iPSCs): the green fluorescence highlights the cytoskeletal element, cytokeratin-18, while the red depicts the nuclear transcription factor, hepatocyte nuclear factor 4 alpha. The use of 3-D culture systems, along with patient derived iPSCs, might soon allow researchers to model the progression of complex liver diseases in vitro, reducing the burden on animal models and paving the way for high-throughput screening of drug candidates in a reproducible and physiologically relevant setting. This image was taken using a Zeiss LSM 700 at the Laboratory for Regenerative Medicine.
Jumping spiders can jump with low take-off angles even from smooth surfaces to hunt prey, escape from predators, or move in challenging terrain. They use adhesive structures under their feet to strengthen their foot contact in order to generate enough friction on smooth surfaces for forward jumps. I investigate how spiders use their adhesive structures to stick during the acceleration phase and detach without too much deceleration. This image of a jumping spider (Pseudeuophrys lanigera) during take-off from a glass coverslip was recorded using a Phantom V7 high-speed camera recording at 4700 images per second.